Interferon Production by Human Cells In Vitro

Interferon production by human leukocytes in vitro: some biological characteristics.

Interferon was optimally produced in human peripheral leukocyte cultures incubated for approximately 19 hr in the presence of Sendai virus at a multiplicity of 10 to 50 EID(50)/cell. For determining whether deoxyribonucleic acid (DNA) synthesis per se was essential for interferon production, 1-beta-D-arabinofuranosylcytosine (Ara-C), a potent DNA inhibitor was studied for its effect on interfer...

Production of human lymphoblastoid interferon by Namalva cells.

Optimum conditions for growth and interferon production by a human lymphoblastoid cell line, Namalva, have been studied. Adaptation to large-scale production is possible utilizing either Sendai virus or Newcastle disease virus. Priming of cultures before induction is unnecessary. The interferon produced has properties similar to human leukocyte interferon. The production of lymphoblastoid inter...

Interferon Production by Mouse Leukocytes in Vitro and in Vivo

Vaccinia virus penetrates, or is phagocytosed by, mouse leukocytes in vitro. A cytotoxic effect is observed, but no new infectious virus is produced. Vaccinia virus, as infectious particles, is eliminated from a culture of leukocytes at a more rapid rate than can be accounted for by thermal inactivation. Leukocytes infected with vaccinia virus produce a substance with the properties of interfer...

In vitro interleukin 4 and interferon-gamma production by mononuclear cells from atopic dermatitis patients

In order to elucidate further the possible role of specific cytokines in the pathogenesis of atopic dermatitis (AD) the in vitro production of interleukin 4 (IL-4) and interferon-gamma (IFN-gamma) in patients with severe atopic dermatitis (n = 4) was compared with that in a group of non-atopic healthy controls. Overall IL-4 production by PHA- and PWM-driven PBMNCs was increased in controls duri...

Sildenafil Effect on Nitric Oxide Secretion by Normal Human Endometrial Epithelial Cells Cultured In vitro